A avaliação da eficiência da Luz Ultravioleta C como método de desinfecção das próteses oculo palpebrais: estudo in vitro.

Resumo (EN)

The goal of this study was to assess the efficacy of ultraviolet-C light emitted by light emitting diodes (UV-C LED) in disinfecting real prototypes of oculo-palpebral prostheses made of medical silicone (A-588-1; Factor II). Twenty-four samples were made using an oculo-palpebral prosthesis model. Subsequently, they were contaminated for twenty-four hours in a pool of microorganisms simulating a real clinical situation. The samples were divided into four groups, namely: no treatment; 0.12% chlorhexidine; UV-C LED; and dimethyl sulfoxide (blank control), and submitted to the proposed treatments. The cell viability of the microorganisms was measured using the tetrazolium salt assay.The optical densities were statistically analyzed. Generalized linear models were used to perform thestatistical analysis. Cell viability results indicated statistically significant differences between groups (p<0.0001), with microbial reduction after exposure to UV-C LED irradiation, in comparison to the control group. It was concluded that treatment with UV-C LED decreased the in vitro microbial cell viability of facial prostheses made of medical silicone.