Perfil microbiológico e proteômico na região transcutânea de implantes extraorais em diferentes condições clínicas.

Resumo (EN)

Objective: The purpose of this study was to investigate the microbiological, proteomic and immunological response profiles of patients in the skin around extraoral implants, used for retaining facial prostheses, in health and disease conditions.
Methods: Twelve patients were selected for this study according to the criteria described by Holgers et. al.1988, for collection of samples in the transcutaneous region of the abutment and implant, one in health condition (grades 0 and 1) and the other in disease condition (grades 2, 3 and 4). A total of 24 samples were collected, 12 in the Disease group and 12 in the Health group. The microbiological profile of the samples was evaluated through DNA extraction and sequencing. For this study, the 16S primer by Illumina Miseq 2x250bp was used, and the V3-V4 region of the gene was selected. Analyzes were performed using bioinformatics tools (QIIME2-2020, demultiplexed FASTQ files, METAGENOm, BIOsource, EBio), for bacterial identification. Proteomic analysis was performed using liquid chromatography coupled to mass spectrometry (UHPLC-MS/MS), and statistical analyzes were performed using the Metaboanalyst software.
Results: The results of the microbiome identified alpha and beta diversity in the microbial community in both groups, and in the disease group there was an abundance of Streptococcus Intermediuns, Corynebacterum Diphtheriae and Prevotella Bivia in relation to the health group. The proteomic analysis showed a differentiation of proteins between the two groups, with greater richness and abundance in the health group in relation to the disease group.
Conclusion: The investigation of the studied profiles showed differences between the groups in the microbiome and proteome that can answer questions related to the difference in clinical behavior and that impact the success of rehabilitation. The bacterial growth in abundance and diversity in the disease group was an evident factor, responsible for the differentiation of the groups, related to the specific point of differentiation of the proteins between the groups and directly influencing the immunological response of the patients. New studies with larger samples should be carried out to correlate the impact of these findings on peri-implant conditions and success of osseointegration.