Ação de proteases e carboidrases na desfloculação de Saccharomyces cerevisae

Resumo (EN)

Objective – To present the study of proteases and carbohydrases in deflocculation of Saccharomyces cerevisiae. Methods– The enzymes were tested in different concentrations, time and pH, and de deflocculation ability of enzumes verified by spectrophotometry. Results –The results showed to require 4650UE of protease 642 in 175 min of reaction as 0.2UE of carbohydrase SP299 for 16h, so that deflocculation in pH 3.6 to be occurred. The yeast viability after the treatments did not suffer alteration (99%) demonstrating that the enzymes do not cause injury in the cells. The pH 2.7 showed the best performance of protease 642 and pH 4.0 was the best one for carbohydrase SP299, with maximum deflocculation, similar to the acid treatment pH 2.0. Conclusion – The results of this research suggest studies of alcoholic fermentation using the enzymatic technology in sulfuric acid replace, aiming at future industrial applications.