Dose subabortiva de buchinha-do-norte prejudicou o comportamento e os níveis de dopamina e noradrenalina de ratos Wistar jovens da geração filial

Resumo (EN)

Buchinha-do-norte, or Luffa operculata (L.) Cogn (Cucurbitaceae), is popularly used against sinusitis and is abortive. The present study verified the effects of vertical exposure to the sub-operative dose of the aqueous extract of Buchinha-do-Norte (EBN) in young adult Wistar rats of the filial generation. Pregnant rats were exposed to 1.0 mg/kg EBN or distilled water (1.00 mL/kg) by gavage between GD17 and GD21 days of gestation. The offspring of the group of mothers who received water or who received EBN were identified as control (GC) or experimental (GE). In PND2, the animals were weighted and weighed. In PND60, the rats of the GC and GE branch generation were submitted to or not to stress induced by the New York subway technique (GS or GCS groups). The animals were submitted to behavioral analysis, performed with the open field (CA) and light-dark box (CCE) devices. After decapitation euthanasia, the liver, kidneys and brain were removed for macro and microscopic analysis and for quantification of neurotransmitters in the hypothalamus, frontal cortex and striatum regions. Rats of the parental generation exposed to EBN and their male offspring showed weight gain as well as had more female offspring. Males of the generation submitted to the stress (GES) presented reduction in the frequency of locomotion and the frequency of getting up and showed increased grooming. The levels of vanilmandelic acid, noradrenaline and dopamine in the hypothalamus were reduced in the GES rats, dihydroxyphenylacetic acid had elevated levels in the striatum, while the amount of 5-hydroxyindoleacetic acid increased in the frontal cortex in the GES group. The vertical exposure to the EBN subabortive dose led to an increase in the weight of primigravida rats and their male offspring, as well as the increase in the number of female offspring born; impaired locomotor behavior, altered the concentration of neurotransmitters in rats of the filial generation, although it did not have histologically altered liver, kidneys and encephalon.