Cinética de migração de células do sistema imune após desafio com células de adenocarcinoma mamário 4T1

Resumo (EN)

Several authors have shown that interactions between tumor and non-tumor cells in the place where it develops, can promote both the elimination as its progression. Although several studies have shown the presence of macrophages, T and B lymphocytes in the tumor microenvironment, to date, little is known in relation to migration kinetics of these cells to the site of the tumor develops. Thus, the aim of this work was to determine the recruitment these cells to the tumor microenvironment in breast adenocarcinoma model. For this, BALB/c female mice were subcutaneously injected or not with PBS or with 4T1 mammary adenocarcinoma cells. After 2, 7, 14 and 30 of injection, the animals were euthanized and cells from peritoneal cavities, spleen, spleen, lymph node and injection site were collected and subjected to analysis by flow cytometry for expression of CD45, CD19, CD23, CD11b, CD4. As expected, results showed the presence of immune cells in the first days after tumor implantation. In addition, we demonstrated increase of B-1 lymphocytes in the tumor site and decrease of these cells in the peritoneal cavities after 2 days of tumor inoculation. Taken together, our findings suggest that B-1 lymphocytes are present in the tumor microenvironment of breast adenocarcinoma model. These observations may contribute to the discovery of new targets for prevention or treatment of tumors with high malignant potential.