Leticia Faustino Adolpho, Helena Bacha Lopes, Gileade Pereira Freitas, Denise Weffort, Gabriela Guaraldo Campos Totoli, Ana Carolina Loyola Barbosa, Rahyza Inacio Freire Assis, Karina Gonzales Silverio Ruiz, Denise Carleto Andia, Adalberto Luiz Rosa, Marcio Mateus Beloti.
Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition to mixed cells, to repair bone tissue. Methods: Cell phenotype, proliferation and differentiation were evaluated. Undifferentiated PDLSCs were injected into rat calvarial defects and the new bone was evaluated by μCT, histology and real-time PCR. Results: PDLSCs exhibited a typical mesenchymal stem cell phenotype and HP-PDLSCs showed lower proliferative and higher osteogenic potential than LP-PDLSCs. PDLSCs induced similar bone formation and histological analysis suggests a remodeling process, confirmed by osteogenic and osteoclastogenic markers, especially in tissues derived from defects treated with HP-PDLSCs. Conclusion: PDLSCs induced similar bone formation irrespective of their in vitro osteogenic potential.
