Pesquisa de enterobactérias em chupetas utilizadas por crianças no ensino infantil de Samambaia-DF

Resumo (EN)

Objective – To check the presence of enterobacteries in pacifiers, evaluating the risk of developing infections related to this type of bacteria. Methods – It is an experimental study. They were analyzed in the study twenty samples of pacifiers collected in three institutions of early childhood education located in the city of Samambaia, Federal District. The material was collected by swab moistened with 0.9% sodium chloride, passed around the pacifier nozzle and transported in a tube containing Cary Blair transport medium. Transferred to peptone water, seeded in Macconkey and incubated in a bacteriological oven at 37 °C for 24 and 48 hours. The bacteria were identified by staining of gram, rugai with lysine and indole test. Results – Of the 20 samples collected, nineteen (95%) presented positive bacterial growth, of which 14 (74%) were positive for the presence of enterobacteries and 5 (26%) were not the study group. The species found were Enterobacter spp. in 6 samples (43%), Klebsiella spp. in 5 (36%), Escherichia coli in 2 (14%) and Salmonella spp. in 1 (7%). Conclusions – The pacifiers for being in direct and constant contact with the oral cavity offer adequate conditions for the development of bacteria. Those once contaminated function as reservoir and transmission medium for these microorganisms. In creches there is an agglomeration of children and objects can easily shared between them, pacifiers are a potential source of contamination, favoring the dissemination of potentially pathogenic microorganisms.