Objective – To evaluate vitamin E supplementation effect on pancreatic gene expression of inflammatory markers in rats with alcoholic chronic pancreatitis. Methods – Wistar rats were divided in 3 groups: control, alcoholic chronic pancreatitis without vitamin E supplementation and alcoholic chronic pancreatitis with vitamin E supplementation. Pancreatitis was induced by liquid diet containing ethanol, cyclosporin A (subcutaneously) and cerulean (intraperitonealy). a-tocopherol content in plasma and liver and pancreas histopathologywere analyzed. Gene expression of COX-2, -SMA, TNF- e Pap inflammatory biomarkers were analyzed through the quantitative real time PCR technique, by SYBR Green method. Results – Vitamin E supplementation decreased the transcripts number for COX-2, -SMA, TNF-a genes and increased for Pap gene, an important defense mechanism component against pancreatic injury. Conclusions – Vitamin E supplementation showed anti-inflammatory and beneficial effects on pancreatic gene expression of some inflammation biomarkers in rats with alcoholic chronic pancreatitis, confirming its participation in the inflammatory response mechanisms in the pancreas.