Concentração inibitória mínima e concentração bactericida mínima de três extratos vegetais antibacterianos selecionados da Floresta Amazônica e da Mata Atlântica brasileiras

Resumo (EN)

Introduction – Up to 705 plant extracts from Amazon Rain Forest and Atlantic Rain Forest were screened against bacteria using the microdilution broth assay. In the present work, minimal inhibitory concentration and minimal bactericidal concentration were obtained from the three selected active extracts. Material and Methods – The microdilution broth assay used in the present analysis consists in evaluating small amounts of extract against the bacteria in agar broth medium in 96 well microplates Results – Three organic extracts obtained from Rapanea parvifolia (MY841), Smilax rufescens (SM53) and Ruizterania retusa (VO581) showed antibacterial activity. MY841 and SM53 showed activity against Enterococcus faecalis MIC = 30 mg/ml; MBC = 60 mg/ml and MIC = 80 mg/ml; MBC = 90 mg/ml, respectively) and VO581 showed activity against Staphylococcus aureus (MIC = 140 mg/ml; MBC = 160 mg/ml) and a mild activity against Pseudomonas aeruginosa (MIC = 200 mg/ml; MBC = 380 mg/ml). Conclusions – The three extracts showed important activity against the bacteria, and they are going to be fractionated and the fractions evaluated against the antibacterial model. The major compounds are going to be isolated as well.