Avaliação histológica da dentina de dentes de ratos submetida à ação da BMP – proteína morfogenética associada à hidroxiapatita e colágeno

Resumo (EN)

Introduction – The objective of this research project was to evaluate histologicaly the effects of the BMP application associated to hydroxyapatite in the rats´ dentin teeth. Material and Method –Twenty male Wistar rats’ teeth weighting between three hundred and three hundred fifty grams, were used into this research. The pilot was constituted of four teeth from all these ones, They have a pattern of the preparation depth. In the Group 1 – control – four teeth were extracted to be observed through the histological aspect of natural dentin; in the Group 2, four teeth had received preparation + BMP + hydroxyapatite + collagen followed by sealing with CIV modified-resin (Vitrebond-3M) and after forty-eight hours, they were extracted. In the Group 3, four teeth had received the same preparation and treatment as well as, the ones from the second group. After five days, the extraction was performed. In the Group 4, four teeth had received the same preparation and treatment, as well as, the second group and were extracted after twenty days. After the extractions, the teeth from the second, third and forth groups, were histologicaly analysed. Results – The histologic aspect observed in laminas had shown that there is biocompatibility evidence between the material and the dentin tissue, because it was observed that there wasn´t degenerative alteration in dentin tubules involved in the process on how to prepare its use with the used material. In some cases, it was possible to observe the aspects like dystrophic calcification suggestive of a protection process to face the aggressive material. Conclusions – The results had concluded that the BMP associated to hydroxyapatite is a biocompatible material, that may be used in dentin. It will be necessary to observe about the acting of its material for a longer period in order to certify about the induction of the repaired dentin formation.