Influência das vesículas extracelulares liberadas por células de melanoma na capacidade funcional e no metabolismo da glicose de macrófagos murinos – estudo in vitro

Resumo (EN)

Many studies seek to explore the role extracellular vesicles (EVs) play in tumors due to the nature of their producing cells, as well as the importance they have in intercellular communication. However, the effects EVs exercise on the functional capability of the immune system’s cells are not completely elucidated. Therefore, this study aims at evaluating the influence EVs from murine melanoma cells have on the metabolism and functional capacity of undifferentiated macrophages. To do so, EVs released by B16F10 melanoma murine cells (EVs-mel) were characterized and in vitro assays were performed with primary macrophages obtained from C57BL/6 mice medullary precursors after differentiation. Results obtained in this study demonstrate that macrophages cultivated with different concentrations of EVs-mel had greater glucose uptake, increased expression of iNOS and IL10, significant increase in the production of cytokines TNF-α, IL-6 and IL-10, however no significant differences of its phagocytic capability were obtained. These results are being presented in paper format and are being submitted to the journal Frontiers in Cell and Developmental Biology. Preliminary data also suggests an increase of the ROS production in macrophages that were treated with 7,5 µg/mL of EVs. Data presented in this study shows that primary macrophages treated with EVs-mel tend to present an activation profile more compatible with M1 macrophages, or of inflammatory profile. Thus, these findings are relevant and offer new information on the effect of EVs derived from tumor cells and their likely modulator role in the development of anticancer therapies.