Resumo (EN)
In the present study, the effects of maternal food restriction in the pregnancy of F0 generation combined with hypercaloric diet (HD) in the puberty of F1 generation on astrocyte behavior of F1 and F2 generation were investigated. The astrocyte behavior was examined by assessing the expression of glial fibrillary acidic protein (GFAP) by immunohistochemistry in astrocytes of several central nervous system (CNS) areas including frontal cortex, parietal cortex, nucleus accumbens, periventricular and arcuate nucleus, pons and cerebellar areas (molecular cell layer and granular cell layer) of F1 and F2 generations. In addition, the effects of immune activation by LPS in the F2 generation were observed. In the F1 generation, it was observed a larger GFAP expression in female rats that received HD on puberty than those that received normocaloric diet at the same time. In the male F2 generation, it was observed a significant reduced GFAP expression in both groups of dam’s generation fed with HD, treated or not with LPS, in relation to F2 generation of dam’s fed with normocaloric diet, treated or not with LPS. These data suggest that this attenuation of LPS effect can be occurred by a transgenerational effect of both, maternal food deprivation in pregnancy of F0 generation and HD diet in puberty of F1 generation.
