Resumo (EN)
Bone metabolism is one of the main influencers in the development of new techniques and approaches in both Medicine and Dentistry. Understanding their molecular mechanisms is critical to the success of clinical treatments. Many studies have shown the potential of mesenchymal stem cells (MSCs) in tissue repair and preservation, as they have the capacity for self-renew and differentiation in cell multilineage. In the oral cavity, PDLSCs (periodontal ligament stem cells) can be found in the periodontal ligament and have the potential for osteogenic differentiation. However, before these cells can serve their purposes in clinical applications, further research is needed to understand the mechanisms involved in their osteogenic potential. To this end, this research aims to identify the possible causes of the heterogeneity of osteogenic potentials among PDLCSCs by analyzing and comparing the transcriptional profile of PDLSCs with different steogenic potentials (high and low) - at baseline and also after osteogenic induction in vitro for 10 days - process that will be performed from transcriptome (or RNA-Seq), which consists of the analysis of all the transcripts expressed by the cellular genome.
